 การประชุมวิชาการIn vitro shoot propagation of Curcuma longa L. from rhizome bud explants.ผู้แต่ง: Dr.Piyamat Srirat, Lecturer , Dr.Sarote Sirisansaneeyakul, Professor , Dr.Pramuk Parakulsuksatid, Associate Professor , Dr.Siripatr Prammanee, Associate Professor , Dr.Wirat Vanichsriratana, Associate Professor , การประชุมวิชาการ: |
 หัวเรื่อง:การเพาะเลี้ยงข้าวพันธุ์ขาวดอกมะลิ 105 ในสภาพปลอดเชื้อ ผู้เขียน: สุรินทร์ ปิยะโชคณากุล, Pradit Pongtongkom, นายเผดิม ระติสุนทร, รองศาสตราจารย์ , นางสาวเสาวนีย์ สุพุทธิธาดา, รองศาสตราจารย์, Lertlak Ngernsiri, Punnee Rodrangboon สื่อสิ่งพิมพ์:pdf AbstractCallus from seeds of rice var. Khao Dawk Mali 105 was induced on the modified Murashige and Skoog (MS) agar medium (1962) supplemented with 15 percent coconut water, 1 g/l casein hydrolysate and various concentrations of 2,4-D, NAA and kinetin. The highest percentage of callus formation (62.50%) was obtained when cultured on the medium supplemented with 1 mg/l each of 2,4-D, NAA and kinetin. About 30 percent of these calli were regenerated to plantlets on the medium containing 2 mg/l kinetin. Seeds cultured on the modified MS agar medium supplemented with 15 percent coconut water and various concentrations of BAP and GA3 produced more than 20 shoots on the medium with 10 gm/l BAP. After transferring these shoots to the modified MS agar medium without growth regulators roots were induced and subsequently developed to plantlets. About 90 percent of these plantlets could survive after transplanting to the greenhouse. |
หัวเรื่อง:ผลของรังสีแกมมาร่วมกับการชักนำให้เกิดยอดจำนวนมากต่อการเปลี่ยนแปลงลักษณะทางพืชไร่บางอย่างของข้าวหอม (Oryza sativa L.) พันธุ์ขาวดอกมะลิ 105 ผู้เขียน:ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์ , Pudcharaporn Tangman, นายวิทยา แสงแก้วสุข, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractMutation induction of aromatic rice (Oryza sativa L.) was conducted after in vitro multiple shoot induction and gamma irradiation. Seeds of aromatic rice variety Khao Dawk Mali 105 (KDML 105) were irradiated by gamma radiation at the doses of 0, 4, 8, 12, 16, 20, 24, 28, 32, 36 and 40 krad. After irradiation, the multiple shoots were induced from the seeds. The shoots were rooted and the plantlets (M subscript 1 R subscript 1) were grown under greenhouse condition until maturity. It was found that at the lower doses the height of M subscript 1 R subscript 1 plants increased and subsequently reduced at the higher doses. The doses from 4 to 20 krad caused a reduction in the number of days to flowering compared with the other doses. Number of tillers and panicles per plant increased as the dose increased. At the doses of 8 and 12 krad the number of fertile panicles per plant increased and gradually decreased at the doses higher than 12 kard. The percentage of seed fertility decreased as the dose increased. The 1,000 grain weight varied depending on the dose. Chlorophyll variation was observed among M subscript 1 R subscript 1 plants derived from the cultures of non-irradiated and irradiated seeds. In M subscript 2 R subscript 2 generation, plant height, number of tillers, panicles and fertile panicles per plant varied depending on the doses (0 to 36 krad) compared to control which grown directly from seeds of KDML 105. However, the percentage of fertile seeds still decreased when the dose increased. Chlorophyll mutation was also found in M subscript 2 R subscript 2 progenies. It is of interest that the M subscript 2 R subscript 2 progenies derived from cultures of non-irradiated and irradiated seeds were photoperiod insensitive. They flowered when they reached the maximum vegetative growth stage without the influence of short daylength. On the contrary the control grown directly from seeds of the photoperiod sensitive KDML 105, flowered after stimulation of short daylength. The inheritance of photoperiod insensitive character in M subscript 3 R subscript 3 generation will be investigated later. If the photoperiod insensitive character expressed in the M subscript 2 R subscript 2 progenies is heritable, it will be useful for selection of pure line KDML 105 for photoperiod insensitivity. |
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หัวเรื่อง:การปรับปรุงข้าวหอม (Oryza sativa L.) พันธ์ขาวดอกมะลิ 105 โดยการใช้เทคนิคการชักนำให้คัพภะสร้างยอดจำนวนมากร่วมกับรังสีแกมมา ผู้เขียน:ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์, ดร.อรอนงค์ นัยวิกุล, ศาสตราจารย์ , นายวิทยา แสงแก้วสุข, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractBreeding of aromatic rice (Oryza sativa L.) variety Khao Dawk Mali 105 (KDML 105) for photoperiod insensitivity, short culm, high yielding, good cooking and eating quality of grains was conducted by using in vitro multiple shoot induction and gamma irradiation. The 357 M1R1 rice plants derived from multiple shoots which were induced from embryos irradiated with various doses of gamma radiation (0-36 krad). The M1R1 plants varied for the number of days to flowering, plant height, number of tillers and panicles per plant, seed fertility and lodging. The 1,108 M2R2 progenies showed photoperiod insensitivity, however the selection for other desirable agronomic characters could not be made due to the progenies which were infected by yellow orange leaf disease. The seeds were harvested from total 371 M2R2 plants which could produce seeds. In M3R3 progenies, selection was made for photoperiod insensitivity, short culm, high tillering, and erect plant type. Ninety-five M3R3 plants were selected. The M4R4 progenies responded to short day length which indicated that they were weakly photoperiod sensitive. The selection was made only for desirable agronomic characters. Thirty-one M4R4 lines were screened. The selection of M5R5 progenies was made by considering both good agronomic characters and good cooking and eating quality of grains. Four M5R5 lines were selected which possessed weakly photoperiod sensitivity, short culm, moderately tillering, erect plant type, good cooking and eating quality of grain with aroma. These lines will be utilized for further breeding of aromatic rice variety KDML 105 for photoperiod insentivity. |
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